- IMAGEJ FOR WINDOWS FOR FREE
- IMAGEJ FOR WINDOWS ZIP FILE
- IMAGEJ FOR WINDOWS ARCHIVE
- IMAGEJ FOR WINDOWS UPGRADE
For an explanation of why overexposure will ruin your results, see this page. This also assumes that you didn’t overexposed your blot image when you produced it. See the references at the end of this tutorial for a discussion of the various ways that you can screw this step up. film), and use the scanner software to save 16-bit tiff images. If you are scanning x-ray film on a flatbed scanner, make sure you use a scanner with the ability to scan transparencies (i.e. tif would be the preferred format to retain the maximum amount of information in the original image). This tutorial assumes that you have carried your gel or blot through the visualization step, so that you have a digital image of your gel in. ImageJ ( ) can be used to compare the density (aka intensity) of bands on an agar gel or western blot.
IMAGEJ FOR WINDOWS FOR FREE
The Image Studio Lite software can be downloaded for free from In addition to the functionality described below for ImageJ, Image Studio Lite provides additional data management features along with annotation tools for producing publication-ready images of your westerns. If you’re looking for a more comprehensive workflow option for your western blot analyses, please visit my tutorial on using Image Studio Lite, a free software package from LI-COR Biosciences.
Plant Proteases and Plant Cell Death: Methods and Protocols. Detection of Damage-Activated Metacaspase Activity by Western Blot in Plants. If you need a peer-reviewed citation for the methods outlined below, you may cite Stael, S., L.P. It can be attached to a shortcut byĬlicking on "Change Icon" in the shortcut's Properties dialog box.The following information is an updated version of a method for using ImageJ to analyze western blots from a now-deprecated older page.
IMAGEJ FOR WINDOWS ZIP FILE
A JARįile is a ZIP file with a different extension.
IMAGEJ FOR WINDOWS UPGRADE
The only file that needs to be replaced to upgrade ImageJ to a newer version. ij.jar - This is a JAR file containing the ImageJ platform independent byte code (class files).If the ImageJ folder is moved to another location. You must edit the "Target" and "Start in" Properties lnk shortcut that runs ImageJ using the JRE (Java Runtime Environment) 1.1.3 in the jre folder. ImageJ does not work very well with Java 1.4.0.This is fixed in JRE 1.3.1_03, now distributed with ImageJ. Printing does not work with the Sun JRE 1.3.1_02 Java runtime.To upgrade to the latest version of ImageJ, replace the ij.jar file in the ImageJ folder with a newer one from. To avoid virtual memory "thrashing" set the -mx option to For example, to increase the available memory from 80MB to 340MB, changeĬ:\ImageJ\jre\bin\javaw.exe -mx340m -cp ij.jar ij.ImageJ ImageJ shortcut, select "Properties", click on the "Shortcut" tab, and edit To increase the amount of memory available to ImageJ, right-click on the ImageJ will run on a machine with 64MB but it runs better with 256MB or more, particularly when working with large images and/or stacks. To create an ImageJ desktop icon, open the ImageJ folder, right-click on the ImageJ shortcut, drag it to the desktop, release the right mouse button, and select "Create Shortcut Here" from the popup menu. (Note: the double quotes are required if there is a space in the target path.) "C:\Program files\ImageJ\jre\bin\javaw.exe" -mx80m -cp ij.jar ij.ImageJ For example, if the ImageJ folder is located in the C:\Program Files folder, change these fields to If this is not the case, right-click on the shortcut and edit the "Target:" and "Start in" fields of the "Shortcut" properties to reflect the new location.
The "ImageJ" shortcut assumes the ImageJ folder is atĬ:\ImageJ. To run ImageJ, open the ImageJ folder and double-click on the ImageJ shortcut (a microscope with an arrow).
IMAGEJ FOR WINDOWS ARCHIVE
Download and expand the self-extracting ZIP archive to the C: drive.